The most notable features of SerialEM 4.0 are:

* Expansion of the Navigator Acquire at Items dialog and capabilities to
  include all commonly run the procedures and to to provide flexibility in how
  often, when in the cycle, and where each is run, thus allowing most SPA
  acquisition to be done without a script.

* New routines and setup dialogs for finding eucentricity from focus,
  aligning to a template, and managing coolant and vacuum microscope events.

* Improvements to the State dialog to allow saving of View and Search states in
  Low Dose mode, and Mont-map states outside of Low Dose.

* Support for Falcon 4 cameras, EER files, and the Selectris filter, as well as
  for saving and aligning frames from Ceta 2 cameras.

* Ability to read and write HDF files, with the "mdoc" metadata included in the
  file.

* Scripting in Python within SerialEM, plus the ability to control SerialEM 
  from a external Python script.

* Other increased scripting capabilities, with 77 new script commands.

* Ability to hide specified menu items and dialog elements, allowing program
  managers to restrict what users can change as well as define a basic mode
  that can be turned on and off.